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Bioinformatics Training

Bioinformatics course timetable

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Tue 17 Jul – Fri 28 Sep

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July 2018

Tue 17
Variant Discovery with GATK4 (2 of 4) In progress 09:30 - 16:30 Bioinformatics Training Room, Craik-Marshall Building, Downing Site

This workshop will focus on the core steps involved in calling variants with the Broad’s Genome Analysis Toolkit, using the “Best Practices” developed by the GATK team. You will learn why each step is essential to the variant discovery process, what are the operations performed on the data at each step, and how to use the GATK tools to get the most accurate and reliable results out of your dataset.

In the course of this workshop, we highlight key functionalities such as the germline GVCF workflow for joint variant discovery in cohorts, somatic variant discovery using MuTect2, and copy number variation discovery using GATK-CNV. All analyses are demonstrated using GATK version 4. Finally, we demonstrate the use of pipelining tools to assemble and execute GATK workflows.

The workshop covers basic genomics, all currently supported Best Practices pipelines as well as pipelining with WDL/Cromwell/FireCloud. This includes the logic of the major pipelines, file formats and data transformations involved, and hands-on operation of the tools using goal-oriented exercises.

  • Day 1: Introduction to Genomics, GATK Best Practices and Pipelining
  • Day 2: Germline short variant discovery (SNPs + Indels)
  • Day 3: Somatic variant discovery (SNVs + Indels + CNVs)
  • Day 4: Writing pipelines with WDL and running them in FireCloud

Please note that this workshop is focused on human data analysis. The majority of the materials presented does apply equally to non-human data, and we will address some questions regarding adaptations that are needed for analysis of non-human data, but we will not go into much detail on those points.

Please note that if you are not eligible for a University of Cambridge Raven account you will need to Book or register Interest by linking here.

Wed 18
Variant Discovery with GATK4 (3 of 4) In progress 09:30 - 16:30 Bioinformatics Training Room, Craik-Marshall Building, Downing Site

This workshop will focus on the core steps involved in calling variants with the Broad’s Genome Analysis Toolkit, using the “Best Practices” developed by the GATK team. You will learn why each step is essential to the variant discovery process, what are the operations performed on the data at each step, and how to use the GATK tools to get the most accurate and reliable results out of your dataset.

In the course of this workshop, we highlight key functionalities such as the germline GVCF workflow for joint variant discovery in cohorts, somatic variant discovery using MuTect2, and copy number variation discovery using GATK-CNV. All analyses are demonstrated using GATK version 4. Finally, we demonstrate the use of pipelining tools to assemble and execute GATK workflows.

The workshop covers basic genomics, all currently supported Best Practices pipelines as well as pipelining with WDL/Cromwell/FireCloud. This includes the logic of the major pipelines, file formats and data transformations involved, and hands-on operation of the tools using goal-oriented exercises.

  • Day 1: Introduction to Genomics, GATK Best Practices and Pipelining
  • Day 2: Germline short variant discovery (SNPs + Indels)
  • Day 3: Somatic variant discovery (SNVs + Indels + CNVs)
  • Day 4: Writing pipelines with WDL and running them in FireCloud

Please note that this workshop is focused on human data analysis. The majority of the materials presented does apply equally to non-human data, and we will address some questions regarding adaptations that are needed for analysis of non-human data, but we will not go into much detail on those points.

Please note that if you are not eligible for a University of Cambridge Raven account you will need to Book or register Interest by linking here.

Thu 19
Variant Discovery with GATK4 (4 of 4) In progress 09:30 - 16:30 Bioinformatics Training Room, Craik-Marshall Building, Downing Site

This workshop will focus on the core steps involved in calling variants with the Broad’s Genome Analysis Toolkit, using the “Best Practices” developed by the GATK team. You will learn why each step is essential to the variant discovery process, what are the operations performed on the data at each step, and how to use the GATK tools to get the most accurate and reliable results out of your dataset.

In the course of this workshop, we highlight key functionalities such as the germline GVCF workflow for joint variant discovery in cohorts, somatic variant discovery using MuTect2, and copy number variation discovery using GATK-CNV. All analyses are demonstrated using GATK version 4. Finally, we demonstrate the use of pipelining tools to assemble and execute GATK workflows.

The workshop covers basic genomics, all currently supported Best Practices pipelines as well as pipelining with WDL/Cromwell/FireCloud. This includes the logic of the major pipelines, file formats and data transformations involved, and hands-on operation of the tools using goal-oriented exercises.

  • Day 1: Introduction to Genomics, GATK Best Practices and Pipelining
  • Day 2: Germline short variant discovery (SNPs + Indels)
  • Day 3: Somatic variant discovery (SNVs + Indels + CNVs)
  • Day 4: Writing pipelines with WDL and running them in FireCloud

Please note that this workshop is focused on human data analysis. The majority of the materials presented does apply equally to non-human data, and we will address some questions regarding adaptations that are needed for analysis of non-human data, but we will not go into much detail on those points.

Please note that if you are not eligible for a University of Cambridge Raven account you will need to Book or register Interest by linking here.

Fri 20
Using the Ensembl Genome Browser [Places] 09:30 - 17:30 Bioinformatics Training Room, Craik-Marshall Building, Downing Site

The Ensembl Project provides a comprehensive and integrated source of annotation of, mainly vertebrate, genome sequences. This workshop offers a comprehensive practical introduction to the use of the Ensembl genome browser as well as essential background information.

This course will focus on the vertebrate genomes in Ensembl, however much of what will be covered is also applicable to the non-vertebrates (plants, bacteria, fungi, metazoa and protists) in Ensembl Genomes.

Please note that if you are not eligible for a University of Cambridge Raven account you will need to book or register your interest by linking here.

Mon 30
CRUK: Image Analysis with Fiji [Places] 12:30 - 17:00 eLearning 1 - School of Clinical Medicine

Fiji/ImageJ is a popular open-source image analysis software application. This course will briefly cover introductory aspects of image processing and analysis theory, but will focus on practical sessions where participants will gain hands on experience with Fiji.

This course is run by the CRUK CI Light microscopy core facility.

Please note that if you are not eligible for a University of Cambridge Raven account you will need to Book or register Interest by linking here.

September 2018

Mon 3
Introduction to RNA-seq data analysis (1 of 3) [Places] 09:30 - 17:30 Bioinformatics Training Room, Craik-Marshall Building, Downing Site

The aim of this course is to familiarize the participants with the primary analysis of RNA-seq data.

This course starts with a brief introduction to RNA-seq and discusses quality control issues. Next, we will present the alignment step, quantification of expression and differential expression analysis. For downstream analysis we will focus on tools available through the Bioconductor project for manipulating and analysing bulk RNA-seq.

Please note that if you are not eligible for a University of Cambridge Raven account you will need to book or register your interest by linking here.

Tue 4
Introduction to RNA-seq data analysis (2 of 3) [Places] 09:30 - 17:30 Bioinformatics Training Room, Craik-Marshall Building, Downing Site

The aim of this course is to familiarize the participants with the primary analysis of RNA-seq data.

This course starts with a brief introduction to RNA-seq and discusses quality control issues. Next, we will present the alignment step, quantification of expression and differential expression analysis. For downstream analysis we will focus on tools available through the Bioconductor project for manipulating and analysing bulk RNA-seq.

Please note that if you are not eligible for a University of Cambridge Raven account you will need to book or register your interest by linking here.

Wed 5
Introduction to RNA-seq data analysis (3 of 3) [Places] 09:30 - 17:30 Bioinformatics Training Room, Craik-Marshall Building, Downing Site

The aim of this course is to familiarize the participants with the primary analysis of RNA-seq data.

This course starts with a brief introduction to RNA-seq and discusses quality control issues. Next, we will present the alignment step, quantification of expression and differential expression analysis. For downstream analysis we will focus on tools available through the Bioconductor project for manipulating and analysing bulk RNA-seq.

Please note that if you are not eligible for a University of Cambridge Raven account you will need to book or register your interest by linking here.

Thu 6
An Introduction to Solving Biological Problems with Python (1 of 2) [Places] 09:30 - 17:30 Bioinformatics Training Room, Craik-Marshall Building, Downing Site

This course provides a practical introduction to the writing of Python programs for the complete novice. Participants are lead through the core aspects of Python illustrated by a series of example programs. Upon completion of the course, attentive participants will be able to write simple Python programs and customize more complex code to fit their needs.

Course materials are available here.

Please note that the content of this course has recently been updated. This course now mostly focuses on core concepts including Python syntax, data structures and reading/writing files. Functions and modules are now the focus of a new 1-day course, Working with Python: functions and modules.

Please note that if you are not eligible for a University of Cambridge Raven account you will need to book by linking here.

Fri 7
An Introduction to Solving Biological Problems with Python (2 of 2) [Places] 09:30 - 17:30 Bioinformatics Training Room, Craik-Marshall Building, Downing Site

This course provides a practical introduction to the writing of Python programs for the complete novice. Participants are lead through the core aspects of Python illustrated by a series of example programs. Upon completion of the course, attentive participants will be able to write simple Python programs and customize more complex code to fit their needs.

Course materials are available here.

Please note that the content of this course has recently been updated. This course now mostly focuses on core concepts including Python syntax, data structures and reading/writing files. Functions and modules are now the focus of a new 1-day course, Working with Python: functions and modules.

Please note that if you are not eligible for a University of Cambridge Raven account you will need to book by linking here.

Thu 27
An Introduction to Machine Learning (1 of 2) [Full] 09:30 - 17:00 Bioinformatics Training Room, Craik-Marshall Building, Downing Site

Machine learning gives computers the ability to learn without being explicitly programmed. It encompasses a broad range of approaches to data analysis with applicability across the biological sciences. Lectures will introduce commonly used algorithms and provide insight into their theoretical underpinnings. In the practicals students will apply these algorithms to real biological data-sets using the R language and environment.

Please be aware that the course syllabus is currently being updated following feedback from the last event; therefore the agenda below will be subjected to changes.

Please note that if you are not eligible for a University of Cambridge Raven account you will need to book by linking here.

Fri 28
An Introduction to Machine Learning (2 of 2) [Full] 09:30 - 17:00 Bioinformatics Training Room, Craik-Marshall Building, Downing Site

Machine learning gives computers the ability to learn without being explicitly programmed. It encompasses a broad range of approaches to data analysis with applicability across the biological sciences. Lectures will introduce commonly used algorithms and provide insight into their theoretical underpinnings. In the practicals students will apply these algorithms to real biological data-sets using the R language and environment.

Please be aware that the course syllabus is currently being updated following feedback from the last event; therefore the agenda below will be subjected to changes.

Please note that if you are not eligible for a University of Cambridge Raven account you will need to book by linking here.



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Training events

September 2018

13 13
MuG API Course
European Bioinformatics Institute (EMBL-EBI) - Wellcome Genome Campus, Hinxton, Cambridge,  CB10 1SD, United Kingdom
13th Sep

The Multiscale complex Genomics (MuG) Virtual Research Environment (VRE) is a workspace designed for researchers to analyse their 3D/4D genomic data. The aim of the course is to provide developers within the life sciences training to be able to wrap their own analysis tools so that it can be easily integrated into the VRE.

First come, first served

October 2018

10 10
ENA Sequence Retrieval
European Bioinformatics Institute (EMBL-EBI) - Wellcome Genome Campus, Hinxton, Cambridge,  CB10 1SD, United Kingdom
10th Oct

This training will introduce ENA (European Nucleotide Archive) users to powerful search and retrieval methods available for annotated sequences, genomes, raw data and metadata, as well as download options from the ENA browser and an introduction to the various ENA browser tools. There will also be a section on cross references and how to retrieve accessions for other resources linked to the primary data in ENA. There will be time for users to present their own use cases or request specific help with their own queries.

First come, first served

15 15
Bioinformatics & Functional Genomics in Zebrafish
European Bioinformatics Institute (EMBL-EBI) - Training Room 1 - Wellcome Genome Campus, Hinxton, Cambridge,  CB10 1SD, United Kingdom
15th 18th Oct

This course has been designed specifically for researchers working within the Zebrafish community, providing an introduction and practical knowledge of tools and resources for working with functional genomics data.

Open application with selection

15th 17 17
Ensembl browser workshop, EMBL-EBI, 17 October 2018
European Bioinformatics Institute (EMBL-EBI) - Wellcome Genome Campus, Hinxton, Cambridge,  CB10 1SD, United Kingdom
17th Oct

Work with the Ensembl Outreach team to get to grips with the Ensembl browser, accessing gene, variation, comparative genomics and regulation data, and mine these data with BioMart.

First come, first served

23 23
Analysis of High Throughput Sequencing Data
European Bioinformatics Institute (EMBL-EBI) - Wellcome Genome Campus, Hinxton, Cambridge,  CB10 1SD, United Kingdom
23rd 26th Oct

This course will familiarise participants with methodologies for bioinformatics analysis on next generation sequencing (NGS) data.

Open application with selection

23rd

November 2018

6 6
Bioinformatics for Plant Biology
European Bioinformatics Institute (EMBL-EBI) - Wellcome Genome Campus, Hinxton, Cambridge,  CB10 1SD, United Kingdom
6th 9th Nov

A course aimed at introducing those working in plant biology to a range of bioinformatics resources and approaches applicable to their research.

Open application with selection

6th 13 13
Exploring Biological Sequences
European Bioinformatics Institute (EMBL-EBI) - Wellcome Genome Campus, Hinxton, Cambridge,  CB10 1SD, United Kingdom
13th 15th Nov

This course will introduce you to approaches used for finding, analysing and using biological sequences.

First come, first served

13th

September 2019

23 23
Introduction to Next Generation Sequencing
European Bioinformatics Institute (EMBL-EBI) - Wellcome Genome Campus, Hinxton, Cambridge,  CB10 1SD, United Kingdom
23rd 27th Sep

This course will provide an introduction to the technology, data analysis, tools and resources for next generation sequencing (NGS) data.

23rd

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CSED 2015 programme

CSED page


COMPUTING FOR RESEARCH
Introduction to NVivo
An Introduction to R  [NEW]
Intermediate Workshop on R [NEW]
An Introduction to PASW (SPSS) [NEW]
Basic Data Analysis PASW (SPSS) [NEW]
Advanced Data Analysis I PASW (SPSS) [NEW]
Advanced Data Analysis II PASW (SPSS) [NEW]
Introduction to Linux [NEW]
Introduction to High Powered Computing [NEW]
Matlab (Online Service) [NEW]

 

Introduction to NVivo

DATE & TIME: Tuesdays 18 & 25 November 2014, 09.30-12.30
REPEATED: Wednesdays 4 & 11 February 2015, 13.30-16.30
REPEATED: Fridays 8 & 15 May 2015, 09.30-12.30
TUTOR: Steve Oldfield, CSED
DURATION: Two half days
VENUE: CSED IT Training Room, 02.01, Library
DETAILS: Nvivo is a software package which makes the task of analysing qualitative data easier. ITCS has arranged a site licence which some Schools at UEA have bought into. It is expected that we will be using version 10 for this course. This course provides a step-by-step introduction to using NVivo for those with little or no experience of it (though some familiarity with analysing qualitative data will be an advantage). Demonstration data will be used, though the techniques and organising principles can be applied to any qualitative data. The course focuses on basic use of the software package; it is not a research methods course.
ADDITIONAL INFORMATION: Participants are expected to attend both parts. A general familiarity with the use of Windows features such as menus, toolbars and dialogue boxes is a prerequisite. Course materials are provided in-session.

[Go to Top of Page]     [Online Course Booking Form]


An Introduction to R [NEW]

DATE & TIME: Wednesday 8 October 2014, 12.00-14.00
VENUE: Room 0.24, Chancellors Drive Annexe
REPEATED: Wednesday 18 February 2015, 12.00-14.00
VENUE: ARTS 1.02
SESSION LEADERS:
Gareth Janacek, CMP
DURATION: 2 hours
RDF Domains: A1, A3
DETAILS: This basic introduction to R aims to get you up and running. We assume some very basic computer skills and a familiarity with data. The topics we expect to cover are;

  • What is R?
  • Basics
  • Preparing and Importing Your Data
  • The Help Files
  • Finding and loading R Packages
  • Graphics
  • Record Keeping
  • Problems/Getting help
  • Examples of analyses: regression and generalised linear models
  • Multivariate techniques (if time permits)

ADDITIONAL INFORMATION: Please feel free to bring your own laptop. The data examples used will be available on Blackboard.

[Go to Top of Page]     [Online Course Booking Form]


Intermediate Workshop on R [NEW]

DATE & TIME: Tuesday 11 November 2014, 12.00-14.00
VENUE: BIO 0.12
REPEATED: Wednesday 13 May 2015, 12.00-14.00
VENUE: ARTS 1.02
SESSION LEADER: Gareth Janacek, CMP
DURATION: 2 hours
RDF Domains: A1, A3
DETAILS: The aim of this session is to show how one can use R to perform statistical analyses. This is a huge subject area and we shall aim to show what is available without delving too far into de   tails. Do feel free to bring your own laptop. The approach is example driven and quite practical.

We would aim to cover:

  • Multivariate analysis
  • PCA
  • Factor Analysis
  • Discriminant analysis
  • Time Series Analysis
  • Time series class
  • Plots
  • Time domain modelling – Forecasting
  • The frequency domain
  • Graphics
  • The basic graphic system, devices and output
  • Trellis graphics
  • GGplot
  • Bayesian Methods
  • Introduction and the LearnBayes pac  kage
  • MCMC
  • R functions for Bayesian inference using lm, glm, mer and polr objects.
  • Point out packages that link R to other Bayesian sampling engines such as JAGS , OpenBUGS , and WinBUGS
  • Survival Analysis
  • Censoring
  • Kaplan –Meier
  • Cox Models

We aim to have at least one example data set in each case.

ADDITIONAL INFORMATION: Feel free to bring your own laptop

[Go to Top of Page]     [Online Course Booking Form]


Introduction to PASW (SPSS) [NEW]

DATE & TIME: Wednesday 14 January 2015, 09.30-12.30
VENUE: Arts, 1.02
SESSION LEADER: Simon Poulton, BIO
DURATION: A half day
RDF Domains: A1, A3
DETAILS:This session introduces you to the PASW (SPSS) statistical software and will cover ways to create and interpret histograms, bar charts and scatter plots using the interactive “Chart Builder”, as well as two main themes;

  • Getting started, including an introduction to the PASW environment (windows & settings), definition of variables, opening and saving PASW files and importing data from other software packages.
  • Exploring your data, including how to create summary statistics for your variables and the creation of frequency tables and cross-tabulations between variables.

ADDITIONAL INFORMATION: There are no attendance requirements for this session, prior knowledge of PASW (SPSS) is not essential, and you do not have to attend all four sessions.  The emphasis is on practical use of PASW rather than statistical theory.  The session will have a strong graphical emphasis and include ‘hands-on’ exercises as well as opportunities for feedback and Q&As.

[Go to Top of Page]     [Online Course Booking Form]


Basic Data Analysis PASW (SPSS) [NEW]

DATE & TIME: Thursday 15 January 2015, 09.30-12.30
VENUE: BIO, 0.12
SESSION LEADERS: Simon Poulton, BIO
DURATION: A half day
RDF Domains: A1, A3
DETAILS: This session assumes that you have used PASW before and have a basic understanding of statistical analysis and testing methods. It will continue the practical use of PASW and show you how to manage, extract and analyse data as well as generate graphical techniques provided in the “Chart Builder”.
ADDITIONAL INFORMATION: To benefit from this session you should be familiar with using PASW.  If you understand and have successfully completed statistical analyses such as ANOVA and linear regression then this course may not be suitable. The emphasis is on practical use of PASW rather than statistical theory. The session will have a strong graphical emphasis and include ‘hands-on’ exercises as well as opportunities for feedback and Q&As.

[Go to Top of Page]     [Online Course Booking Form]

 


Advanced Data Analysis I PASW (SPSS) [NEW]

DATE & TIME: Wednesday 11 March 2015, 09.30-12.30
VENUE: Arts, 1.02
SESSION LEADER: Simon Poulton, BIO
DURATION: A half day
RDF Domains: A1, A3
DETAILS: This session will introduce the concepts of Analysis-of-Variance, Linear Regression and General Linear Models, how they are run and interpreted in PASW. It will cover advanced PASW data handling methods; transposing data and the comprehensive two-stage process for data validation, including the detection of outliers. It introduces the concepts of ANOVA, then moves forward to two-way and multi-way ANOVA, with particular emphasis on how to arrange your data and explore methods of correlation and multiple regression models.
ADDITIONAL INFORMATION: To benefit from this session you should be well-versed in the use of PASW (SPSS) or other statistical software and confident in manipulating data, although perhaps unsure how to achieve this in PASW. You will require an understanding of concepts such as the sums-of-squares and variances, confidence intervals and how to interpret p-values. If you possess extensive experience in using GLMs for linear modelling, then this course may not be suitable. The emphasis is on practical use of PASW rather than statistical theory. The session will have a strong graphical emphasis and include ‘hands-on’ exercises as well as opportunities for feedback and Q&As.

 

[Go to Top of Page]     [Online Course Booking Form]

 


Advanced Data Analysis II PASW (SPSS) [NEW]

DATE & TIME: Wednesday 27 May 2015, 09.30-12.30
VENUE: Arts, 1.02
SESSION LEADER: Simon Poulton
DURATION: A half day
RDF Domains: A1, A3
DETAILS: This session continues the theme of practical use of the software. It explores the importance of residuals and diagnostic tests in ANOVA and regression models and will also cover a brief introduction to Analysis-of-Covariance (ANCOVA). Emphasis will be on how to organise your data and specify models using the GLM dialogue boxes. It will explore the use of mixed models and repeated-measures models and outline how they are implemented and defined in PASW. If you are curious about residuals plots, post-hoc testing or more complex types of linear models then this session may be for you. There will be a strong emphasis on the interpretation of PASW outputs.
ADDITIONAL INFORMATION: You should possess considerable experience of manipulating data and an understanding of basic linear modelling techniques. Familiarity with interpreting the outputs from simple ANOVA and regression models is also required. The emphasis is on practical use of PASW rather than statistical theory. The session will have a strong graphical emphasis and include ‘hands-on’ exercises as well as opportunities for feedback and Q&As.

[Go to Top of Page]     [Online Course Booking Form]


Introduction to Linux [NEW]

RDF Domains: A1, A3
DETAILS: Linux is a powerful and versatile open source operating system frequently found powering desktop computers and servers, including the UEA High Performance Computing Cluster. In one-to-one or small group sessions we will work through the fundamental skills needed to begin working with Linux tailored to the individual: from familiarising participants with the working environment, using common commands and file management to more advanced usage and scripting.
ADDITIONAL INFORMATION: This course is held on a one-to-one basis. For further information and to register your interest, please contact hpc.admin@uea.ac.uk

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Introduction to High Powered Computing [NEW]

RDF Domains: A1, A3
DETAILS: Do you have a computational element to your research? In a one-to-one session we will introduce UEA’s HPC cluster and discuss how it can benefit your research looking at your own specific computational requirements. We will go through the initial steps on using the HPC cluster and help you get started with your own task.
ADDITIONAL INFORMATION: This course is held on a one-to-one basis. For further information and to register your interest, please contact hpc.admin@uea.ac.uk

[Go to Top of Page]


Matlab (Online Service) [NEW]

RDF Domains: A1, A3
DETAILS:

Matlab Fundamentals

This online course provides a comprehensive introduction to the MATLAB technical computing environment and is intended for beginners and those looking for a review. No prior programming experience or knowledge of MATLAB is necessary. The course is structured to allow thorough assimilation of ideas through hands-on examples and exercises. MATLAB competency is developed in a natural way, with an emphasis on practical application. Themes of data analysis, visualization, modelling, and programming are explored throughout the course.

Topics include:

  • Working with the MATLAB user interface
  • Entering commands and creating variables
  • Performing analysis on vectors and matrices
  • Visualizing vector and matrix data
  • Working with data files
  • Working with data types
  • Automating commands with scripts
  • Writing programs with logic and flow control
  • Writing functions

Matlab for Data Processing and Visualisation

This course focuses on the details of data management and visualization techniques, from reading arbitrarily formatted text data files to producing customized publication-quality graphics. The course emphasizes creating scripts that extend the basic features provided by MATLAB. Topics include:

  • Importing data
  • Organizing data
  • Visualizing data

Matlab Programming Techniques

This course provides hands-on experience using the features in the MATLAB® language to write efficient, robust, and well-organized code. These concepts form the foundation for writing full applications, developing algorithms, and extending built-in MATLAB capabilities. Details of performance optimization, as well as tools for writing, debugging, and profiling code, are covered. Topics include:

  • Creating robust applications
  • Utilizing development tools
  • Structuring code
  • Structuring data
  • Efficient data management
  • Classes and objects

ADDITIONAL INFORMATION: The Matlab sessions are available to take as an online service. For further information please contact hpc.admin@uea.ac.uk

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